Cold-Stored Mycelial Transfer - Petri, Agar, and Transfer Methodolo...

Welcome to my summary of this weeks work on petri dish utilization via transfer and isolation of cold-stored psilocybin multi-strained dishes!

This may sound confusing, but, simply put, there were three days of work and essentially three major steps to this process:

Clean, Sanitize, and Sterilize Dishes
Prepare and Pour Agar Agar, and
Cut cold-stored Petri storage colonies to these newly prepared Agar Petri Dishes.

So, without further adieu, let's begin!

Wednesday, January 12th 2022

This day was spent preparing my dishes for cleaning.

After receiving,

I prepared my work station,

With utilization of Clorox after a soap and water clean and rinse for both 2 minutes, 99% Isopropyl Alcohol is used to soak the dish for 15 seconds.

The dishes cleaned well, and were ready after a nights dry to be used tomorrow!

Yay! Past Section 1.1.1 and 2 of my tek, this is seen in Section 1.2: - @trezzahn/mycelial-spore-utilization-on-agar-petri-dishes-p2-4-in-a-two-blog-series

Thursday, January 13th 2022

Onward! Today I prepared Agar! I used my reagent ingredient recipe found here - @trezzahn/an-introduction-to-agar-and-its-utilization-working-with-it-proper-technique-and-further-use-p2-4-in-a-two-blog-series
And following proper procedure, stove-top cooked my Agar Agar recipe to create Agar -

Such for use pouring in my own now dried Petri dishes -

And letting these now poured Agar dishes cool overnight until the following day!

Friday, January 14th 2022

Lastly, these now poured Agar Dishes are cooled and ready for use!

These are prepared for use transferring previously cold-stored Petri's to these new dishes - something done every 6 months to ensure viability -

And as these prepared dishes are cut,

according to my tek Section 1.3.2 found here - @trezzahn/mycelium-growth-preparation-agar-additionals-general-timeframes-and-further-work-the-fun-part
I transfer, beautifully, two Golden Mammoth - Ajax, two Golden Capped - Penis Envy and One P. Cyanescens to these five prepared Agar Petri's;